Proteintech, 28925-1-AP, Phospho-PLA2G4A (Ser505) Polyclonal antibody

Size: 20ul / 100ul The Phospho-PLA2G4A (Ser505) (28925-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-PLA2G4A (Ser505) in WB, IF/ICC, ELISA applications with reactivity to Human samples 28925-1-AP targets Phospho-PLA2G4A (Ser505) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: λ phosphatase treated HeLa cells Positive IF/ICC detected in: λ phosphatase treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PLA2G4A, also named as cPLA2 (cytosolic phospholipase A2), is a highly conserved and widely expressed enzyme which can promote lipid mediator production. PLA2G4A plays a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. It has been reported that the activity and expression levels of PLA2G4A are upregulated in several neurodegenerative diseases. PLA2G4A can be activated at the phosphorylation site of ser505 by MAP kinase. (PMID: 25838312, 8381049, 31238788) Specification Tested Reactivity: Human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: phospholipase A2, group IVA (cytosolic, calcium-dependent) Calculated Molecular Weight: 749 aa, 85 kDa Observed Molecular Weight: 100-110 kDa GenBank Accession Number: BC114340 Gene Symbol: PLA2G4A Gene ID (NCBI): 5321 RRID: AB_2918213 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P47712 Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, and 0.05% BSA, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage.