Proteintech, 28977-1-AP, Phospho-STAT1 (Ser727) Polyclonal antibody

Size: 20ul / 100ul The Phospho-STAT1 (Ser727) (28977-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-STAT1 (Ser727) in WB, IF/ICC, ELISA applications with reactivity to human samples 28977-1-AP targets Phospho-STAT1 (Ser727) in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: IFN gamma treated HeLa cells Positive IF/ICC detected in: IFN alpha treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT1 activating phosphorylation at Ser727 is p38-dependant during lipotoxic stress. MLK3 activation triggers a MAPK signaling cascade resulting in STAT1 Ser727 phosphorylation. The phosphorylation of STAT1 Ser727 increases its nuclear localization, and transcriptional activity. (PMID: 28262979). Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: signal transducer and activator of transcription 1, 91kDa Calculated Molecular Weight: 83 kDa Observed Molecular Weight: 84 kDa GenBank Accession Number: BC002704 Gene Symbol: STAT1 Gene ID (NCBI): 6772 RRID: AB_2923585 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P42224 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.