Proteintech, 28988-1-AP, Phospho-p70(S6K) (Thr229)/p85(S6K) (Thr252) Polyclonal antibody

Size: 20ul / 100ul The Phospho-p70(S6K) (Thr229)/p85(S6K) (Thr252) (28988-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-p70(S6K) (Thr229)/p85(S6K) (Thr252) in WB, IF/ICC, ELISA applications with reactivity to human samples 28988-1-AP targets Phospho-p70(S6K) (Thr229)/p85(S6K) (Thr252) in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: λ phosphatase treated HeLa cells Positive IF/ICC detected in: λ phosphatase treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K), which is a serine/threonine kinase regulated by phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR) pathway. P70S6K plays a crucial role in controlling cell cycle, growth and survival. The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation and metabolism and is the central regulator of translation of some components of protein synthesis system. Due to alternative translation two isoform S6K1 proteins are known to exist in mammalian cells: p85 S6K1 and p70 S6K1, which is identical to p85 S6K but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1). (PMID: 25100792, PMID: 24970012, PMID: 21602892) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ribosomal protein S6 kinase, 70kDa, polypeptide 1 Calculated Molecular Weight: 59 kDa Observed Molecular Weight: 65-85 kDa GenBank Accession Number: BC053365 Gene Symbol: p70 S6K Gene ID (NCBI): 6198 RRID: AB_2918226 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P23443 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.