Proteintech, 29000-1-AP, Phospho-TSC2 (Thr1462) Polyclonal antibody

Size: 20ul / 100ul The Phospho-TSC2 (Thr1462) (29000-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-TSC2 (Thr1462) in WB, ELISA applications with reactivity to Human, Mouse samples 29000-1-AP targets Phospho-TSC2 (Thr1462) in WB, ELISA applications and shows reactivity with Human, Mouse samples. Tested Applications Positive WB detected in: PDGF treated PC-3 cells, PDGF treated NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information TSC2, also named as Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. It has been reported that TSC2 can be phosphorylated at Thr1462 by Akt/PKB involved in stimulating cell growth and is activated by insulin. (PMID: 14651849, 12172553) Specification Tested Reactivity: Human, Mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: tuberous sclerosis 2 Calculated Molecular Weight: 1807 aa, 201 kDa Observed Molecular Weight: 200 kDa GenBank Accession Number: BC150300 Gene Symbol: TSC2 Gene ID (NCBI): 7249 RRID: AB_2918227 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P49815 Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, and 0.05% BSA, pH 7.3. Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20 o C storage.