Proteintech, 29107-1-AP, Phospho-PKN1 (Thr774) Polyclonal antibody

Size: 20ul / 100ul The Phospho-PKN1 (Thr774) (29107-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-PKN1 (Thr774) in WB, ELISA applications with reactivity to Human, mouse samples 29107-1-AP targets Phospho-PKN1 (Thr774) in WB, ELISA applications and shows reactivity with Human, mouse samples. Tested Applications Positive WB detected in: PDGF treated NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information PKN1 is a stress-responsive kinase and a member of the protein kinase novel (PKN) family also known as protein kinase C-related kinases (PRKs). PKN1 acting downstream of small GTP-binding proteins of the Rho/Rac family. The interaction of Rho with PKN1 induces a conformational change in PKN1 leading to activation loop phosphorylation by PDK1 on Thr774 in the case of human PKN1 (Thr778 in mouse/rat) which is necessary for the catalytic activation of PKN1/2 and critical for the stability of the protein. (PMID: 29045568, PMID: 21037231) Specification Tested Reactivity: Human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: protein kinase N1 Calculated Molecular Weight: 942 aa, 104 kDa Observed Molecular Weight: 130 kDa GenBank Accession Number: BC040061 Gene Symbol: PKN1 Gene ID (NCBI): 5585 RRID: AB_2918235 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q16512 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.