Proteintech, 29208-1-AP, PKC Gamma Polyclonal antibody

Size: 20ul / 150ul The PKC Gamma (29208-1-AP) by Proteintech is a Polyclonal antibody targeting PKC Gamma in WB, IHC, ELISA applications with reactivity to Human, mouse, rat samples 29208-1-AP targets PKC Gamma in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, rat brain tissue Positive IHC detected in: mouse cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14 (SCA14), an autosomal dominant neurodegenerative disease. Specification Tested Reactivity: Human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag25085 Product name: Recombinant human PRKCG protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 600-641 aa of BC047876 Sequence: SGPDGEPTIRAHGFFRWIDWERLERLEIPPPFRPRPCGRSGE Predict reactive species Full Name: protein kinase C, gamma Calculated Molecular Weight: 78 kDa Observed Molecular Weight: 78 kDa GenBank Accession Number: BC047876 Gene Symbol: PKC Gamma Gene ID (NCBI): 5582 RRID: AB_2918249 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P05129 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.