Proteintech, 29259-1-AP, HEXA Polyclonal antibody

Size: 20ul / 150ul The HEXA (29259-1-AP) by Proteintech is a Polyclonal antibody targeting HEXA in WB, IHC, ELISA applications with reactivity to Human, mouse samples 29259-1-AP targets HEXA in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, NIH/3T3 cells, HepG2 cells, Jurkat cells, PC-3 cells Positive IHC detected in: human kidney tissue, human lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Beta-hexosaminidase subunit alpha is involved in catalysis of degradation of the ganglioside GM2. Disfunction of beta-hexosaminidase leads to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. The enzyme is composed of two subunits, alpha and beta. This gene encodes alpha subunit of beta-hexosaminidase. Western blot with this antibody raised against HEXA detected 60 kDa (precursor) and 54 kDa (mature) size bands (PMID: 27682588, PMID: 30341570). Specification Tested Reactivity: Human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag30808 Product name: Recombinant human HEXA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 318-529 aa of BC018927 Sequence: HLGGDEVDFTCWKSNPEIQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYGKGYVVWQEVFDNKVKIQPDTIIQVWREDIPVNYMKELELVTKAGFRALLSAPWYLNRISYGPDWKDFYVVEPLAFEGTPEQKALVIGGEACMWGEYVDNTNLVPRLWPRAGAVAERLWSNKLTSDLTFAYERLSHFRCELLRRGVQAQPLNVGFCEQEFEQT Predict reactive species Full Name: hexosaminidase A (alpha polypeptide) Calculated Molecular Weight: 529 aa, 60 kDa Observed Molecular Weight: 50-54 kDa, 60-67 kDa GenBank Accession Number: BC018927 Gene Symbol: HEXA Gene ID (NCBI): 3073 RRID: AB_2918259 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P06865 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.