Proteintech, 29415-1-AP, PTPRS Polyclonal antibody

Size: 20ul / 150ul The PTPRS (29415-1-AP) by Proteintech is a Polyclonal antibody targeting PTPRS in WB, IHC, ELISA applications with reactivity to human, mouse samples 29415-1-AP targets PTPRS in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, A549 cells, HeLa cells Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information PTPRS, also known as R-PTP-sigma or PTPσ, is a member of the protein tyrosine phosphatase (PTP) family (PMID: 8524829). PTPs dephosphorylate phosphotyrosyl residues in proteins that are phosphorylated by protein-tyrosine kinases (PTKs) (PMID: 20838449). PTPs are essential regulators of signal transduction pathways and play important roles in regulating many cellular processes in conjunction with PTKs (PMID: 7834742). PTPRS contains an extracellular region, a single transmembrane segment and two tandem intracellular catalytic domains, and thus represents a receptor-type PTP. Full-length PTPRS is 217 kDa and has been reported to be proteolytically cleaved into a 140-kDa N-terminal subunit containing extracellular and transmembrane domains and a C-terminal subunit (PMID: 29915291). This antibody raised against 825-1000 aa of human PTPRS detects endogenous PTPRS with an apparent molecular weight of 140 kDa. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag30152 Product name: Recombinant human rPTPσ protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 825-1000 aa of NM_002850 Sequence: VVVTKGAVLGRPTLSVQQTPEGSLLARWEPPAGTAEDQVLGYRLQFGREDSTPLATLEFPPSEDRYTASGVHKGATYVFRLAARSRGGLGEEAAEVLSIPEDTPRGHPQILEAAGNASAGTVLLRWLPPVPAERNGAIVKYTVAVREAGALGPARETELPAAAEPGAENALTLQGL Predict reactive species Full Name: protein tyrosine phosphatase, receptor type, S Calculated Molecular Weight: 217 kDa Observed Molecular Weight: 140 kDa GenBank Accession Number: NM_002850 Gene Symbol: PTPRS Gene ID (NCBI): 5802 RRID: AB_2918301 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13332 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.