Proteintech, 29528-1-AP, Phospho-IRF3 (Ser396) Polyclonal antibody

Size: 20ul / 100ul The Phospho-IRF3 (Ser396) (29528-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-IRF3 (Ser396) in WB, ELISA applications with reactivity to human samples 29528-1-AP targets Phospho-IRF3 (Ser396) in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: LPS treated THP-1 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information Interferons (IFNs) are a large family of multifunctional secreted proteins involved in antiviral defense, cell growth regulation, and immune activation(PMID: 18440553). A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. IRF-3 is activated through phosphorylation by the kinases IKKɛ and/or TBK1. Phosphorylation at Ser396 is essential for IRF-3 activation. After cytoplasmic IRF-3 is phosphorylated at Ser386 and Ser396, phosphorylated IRF-3 translocates into the nucleus. This phosphorylated IRF-3 activates IFN and IFN-responsive genes. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, pig, monkey Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: interferon regulatory factor 3 Calculated Molecular Weight: 47 kDa Observed Molecular Weight: 50-60 kDa GenBank Accession Number: BC009395 Gene Symbol: IRF3 Gene ID (NCBI): 3661 RRID: AB_2935415 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q14653 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.