Product Description
Size: 20ul / 150ul
The FMNL3 (29756-1-AP) by Proteintech is a Polyclonal antibody targeting FMNL3 in WB, IHC, IF/ICC, ELISA applications with reactivity to Human, mouse, rat samples
29756-1-AP targets FMNL3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications
Positive WB detected in: mouse liver tissue, rat liver tissue
Positive IHC detected in: human colon cancer tissue, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: HUVEC cells
Recommended dilution
Western Blot (WB): WB : 1:1000-1:4000
Immunohistochemistry (IHC): IHC : 1:50-1:500
Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
Background Information
FMNL3 plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the control of cell shape and migration. In quiescent endothelial cells, FMNL3 triggers rearrangement of the actin cytoskeleton, but does not alter microtubule alignement.
Specification
Tested Reactivity: Human, mouse, rat
Cited Reactivity: human
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Immunogen: CatNo: Ag30543 Product name: Recombinant human FMNL3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 403-510 aa of NM_175736 Sequence: EHVSHLTEKLLDLENENMMRVAELEKQLLQREKELESIKETYENTSHQVHTLRRLIKEKEEAFQRRCHLEPNVRGLESVDSEALARVGPAELSEGMPPSDLDLLAPAP Predict reactive species
Full Name: formin-like 3
Calculated Molecular Weight: 117 kDa
Observed Molecular Weight: 117 kDa
GenBank Accession Number: NM_175736
Gene Symbol: FMNL3
Gene ID (NCBI): 91010
RRID: AB_3086155
Conjugate: Unconjugated
Form: Liquid
Purification Method: Antigen affinity purification
UNIPROT ID: Q8IVF7
Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.
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Collaboration
Tony Tang
Email: Tony.Tang@iright.com
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