Proteintech, 29771-1-AP, EBP50/NHERF-1 Polyclonal antibody

Size: 20ul / 150ul The EBP50/NHERF-1 (29771-1-AP) by Proteintech is a Polyclonal antibody targeting EBP50/NHERF-1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 29771-1-AP targets EBP50/NHERF-1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HCT 116 cells, HepG2 cells, MCF-7 cells, MDA-MB-231 cells, rat kidney tissue, mouse kidney tissue Positive IHC detected in: human placenta tissue, rat kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, A549 cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information Na+/H+ exchanger regulatory factor 1 (NHERF-1, also known as EBP50 ezrin-binding protein of 50 kDa, SLCA9A3R1) is a phosphoprotein that assembles multiprotein complexes via two PDZ domains and a C-terminal ezrin-binding domain. NHERF-1 was initially recognized as a scaffolding protein that recruits membrane transporters/receptors and cytoplasmic signaling proteins into functional complexes localized at or near the plasma membrane in epithelial cells (PMID: 19308292). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag30878 Product name: Recombinant human SLC9A3R1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 260-358 aa of NM_004252 Sequence: TNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL Predict reactive species Full Name: solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 Calculated Molecular Weight: 39 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: NM_004252 Gene Symbol: SLC9A3R1/EBP50/NHERF-1 Gene ID (NCBI): 9368 RRID: AB_2935478 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O14745 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.