Proteintech, 29814-1-AP, Cytochrome P450 Reductase Polyclonal antibody

Size: 20ul / 150ul The Cytochrome P450 Reductase (29814-1-AP) by Proteintech is a Polyclonal antibody targeting Cytochrome P450 Reductase in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 29814-1-AP targets Cytochrome P450 Reductase in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human placenta tissue, HepG2 cells, mouse liver tissue, rat liver tissue Positive IHC detected in: mouse kidney tissue, rat kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:1500-1:6000 Background Information Cytochrome P450 Reductase (CYPOR) consists of the flavin mononucleotide (FMN) domain and the flavin adenine dinucleotide (FAD) domain, which bind the corresponding cofactors and transfer electrons from NADPH via FAD and FMN (PMID: 33498551). CYPOR is an essential component of the microsomal P450 mixed-function oxidase system and is required for electron transfer from NADPH to cytochromes P450 in microsomes. The calculated molecular weight of CYPOR is 77 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag30899 Product name: Recombinant human POR protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 43-216 aa of NM_000941 Sequence: LFRKKKEEVPEFTKIQTLTSSVRESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEEYDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGVKFAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFELGLGDDDGNLEEDF Predict reactive species Full Name: P450 (cytochrome) oxidoreductase Calculated Molecular Weight: 77 kDa Observed Molecular Weight: 77 kDa GenBank Accession Number: NM_000941 Gene Symbol: Cytochrome P450 Reductase Gene ID (NCBI): 5447 RRID: AB_3086171 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P16435 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.