Proteintech, 29874-1-AP, Phospho-PPP1CA (Thr320) Polyclonal antibody

Size: 20ul / 100ul The Phospho-PPP1CA (Thr320) (29874-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-PPP1CA (Thr320) in WB, ELISA applications with reactivity to Human samples 29874-1-AP targets Phospho-PPP1CA (Thr320) in WB, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: Calyculin A treated HeLa cells, Calyculin A treated Jurkat cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information Protein phosphatase associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is essential for cell division and participates in the regulation of glycogen metabolism, muscle contractility, and protein synthesis. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (PMID: 9835651). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (PMID: 8384581, PMID: 1392080). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (PMID: 9122166). Specification Tested Reactivity: Human Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: protein phosphatase 1, catalytic subunit, alpha isoform Calculated Molecular Weight: 330 aa, 38 kDa Observed Molecular Weight: 37 kDa GenBank Accession Number: BC001888 Gene Symbol: PPP1CA Gene ID (NCBI): 5499 RRID: AB_2935422 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P62136 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.