Proteintech, 29920-1-AP, POLL Polyclonal antibody

Size: 20ul / 150ul The POLL (29920-1-AP) by Proteintech is a Polyclonal antibody targeting POLL in WB, ELISA applications with reactivity to Human samples 29920-1-AP targets POLL in WB, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: L02 cells, HEK-293 cells, HepG2 cells, HEK-293T cells Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Background Information POLL (DNA polymerase lambda) functions in DNA repair (PMID: 11457865, PMID: 19806195, PMID: 20693240, PMID: 30250067). POLL has 5-prime-deoxyribose-5-phosphate lyase activity and strand-displacement synthesis activity on gapped DNA substrates, indicating that POLL participates in short- and long-patch base excision repair. POLL is involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA (PMID: 11457865, PMID: 19806195). Specification Tested Reactivity: Human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag31995 Product name: Recombinant human POLL protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 389-575 aa of BC068529 Sequence: REEATEIEQTVQKAAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLLDSLRQEGFLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVPYSEFACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGCKVGPGRVLPTPTEKDVFRLLGLPYREPAERDW Predict reactive species Full Name: polymerase (DNA directed), lambda Calculated Molecular Weight: 63 kDa Observed Molecular Weight: 63-70 kDa GenBank Accession Number: BC068529 Gene Symbol: POLL Gene ID (NCBI): 27343 RRID: AB_3086189 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UGP5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.