Proteintech, 29934-1-AP, GPR179 Polyclonal antibody

Size: 20ul / 150ul The GPR179 (29934-1-AP) by Proteintech is a Polyclonal antibody targeting GPR179 in WB, IHC, ELISA applications with reactivity to human, mouse samples 29934-1-AP targets GPR179 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells Positive IHC detected in: mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information GPR179 (Probable G-protein coupled receptor 179) belongs to the glutamate receptor subfamily of GPCRs, which is involved in signal transduction through retinal depolarizing bipolar cells. The protein has an EGF-like calcium binding domain and a seven transmembrane domain in the N-terminal region of the protein. Mutations in this gene are associated with congenital stationary night blindness type 1E. GPR179 is expressed in retinal bipolar cells and upregulated in rd1 mice retina with Pde6b mutations (PubMed: 22325361, PubMed: 22325362). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag32180 Product name: Recombinant human GPR179 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 26-381 aa of NM_001004334.3 Sequence: LGGPRPIRSLPPLSSQVKPGSVPMQVPLEGAEAALAYLYSGDAQQLSQVNCSERYEARGAGAMPGLPPSLQGAAGTLAQAANFLNMLLQANDIRESSVEEDVEWYQALVRSVAEGDPRVYRALLTFNPPPGASHLQLALQATRTGEETILQDLSGNWVQEENPPGDLDTPALKKRVLTNDLGSLGSPKWPQADGYVGDTQQVRLSPPFLECQEGRLRPGWLITLSATFYGLKPDLSPEVRGQVQMDVDLQSVDINQCASGPGWYSNTHLCDLNSTQCVPLESQGFVLGRYLCRCRPGFYGASPSGGLEESDFQTTGQFGFPEGRSGRLLQCLPCPEGCTSCMDATPCLVEEAAVLR Predict reactive species Full Name: G protein-coupled receptor 179 Calculated Molecular Weight: 257 kDa Observed Molecular Weight: 260-270 kDa GenBank Accession Number: NM_001004334.3 Gene Symbol: GPR179 Gene ID (NCBI): 440435 RRID: AB_2923622 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6PRD1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.