Proteintech, 29975-1-AP, Uromodulin Polyclonal antibody

Size: 20ul / 150ul The Uromodulin (29975-1-AP) by Proteintech is a Polyclonal antibody targeting Uromodulin in WB, IHC, IF-P, ELISA applications with reactivity to Human, Mouse samples 29975-1-AP targets Uromodulin in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse samples. Tested Applications Positive WB detected in: human urine sample Positive IHC detected in: human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse kidney tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:5000-1:20000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information Uromodulin, also known as THP (Tamm-Horsfall glycoprotein), is the most abundant protein excreted in the urine under physiological conditions. Uromodulin is synthesized by the kidney and localizes in cells lining the ascending limb of Henle and distal convoluted tubule. Uromodulin has been linked to water/electrolyte balance and to kidney innate immunity. Also, studies in knockout mice demonstrated that it has a protective role against urinary tract infections and renal stone formation. Mutations in the gene encoding uromodulin lead to rare autosomal dominant diseases, collectively referred to as uromodulin-associated kidney diseases. (PMID: 21654721) Specification Tested Reactivity: Human, Mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag32343 Product name: Recombinant human UMOD protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 262-396 aa of BC035975 Sequence: SSVEGTCEECSIDEDCKSNNGRWHCQCKQDFNITDISLLEHRLECGANDMKVSLGKCQLKSLGFDKVFMYLSDSRCSGFNDRDNRDWVSVVTPARDGPCGTVLTRNETHATYSNTLYLADEIIIRDLNIKINFAC Predict reactive species Full Name: uromodulin Calculated Molecular Weight: 640 aa, 70 kDa Observed Molecular Weight: 80-90 kDa GenBank Accession Number: BC035975 Gene Symbol: Uromodulin Gene ID (NCBI): 7369 RRID: AB_2935492 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P07911 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.