Proteintech, 29992-1-AP, Fibulin 5 Polyclonal antibody

Size: 20ul / 150ul The Fibulin 5 (29992-1-AP) by Proteintech is a Polyclonal antibody targeting Fibulin 5 in WB, IHC, ELISA applications with reactivity to Human, mouse, rat samples 29992-1-AP targets Fibulin 5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples. Tested Applications Positive WB detected in: mouse heart tissue, mouse kidney tissue, rat heart tissue Positive IHC detected in: human colon cancer tissue, mouse lung tissue, mouse colon tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Fibulin-5, also known as EVEC and DANCE, is an extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies. Specification Tested Reactivity: Human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag30835 Product name: Recombinant human FBLN5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 30-254 aa of BC022280 Sequence: GFDLDRQSGQCLDIDECRTIPEACRGDMMCVNQNGGYLCIPRTNPVYRGPYSNPYSTPYSGPYPAAAPPLSAPNYPTISRPLICRFGYQMDESNQCVDVDECATDSHQCNPTQICINTEGGYTCSCTDGYWLLEGQCLDIDECRYGYCQQLCANVPGSYSCTCNPGFTLNEDGRSCQDVNECATENPCVQTCVNTYGSFICRCDPGYELEEDGVHCSDMDECSFS Predict reactive species Full Name: fibulin 5 Calculated Molecular Weight: 448 aa, 50 kDa Observed Molecular Weight: 65-70 kDa GenBank Accession Number: BC022280 Gene Symbol: Fibulin 5 Gene ID (NCBI): 10516 RRID: AB_2935495 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UBX5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.