Proteintech, 30201-1-AP, FCGR3B/CD16b Polyclonal antibody

Size: 20ul / 150ul The FCGR3B/CD16b (30201-1-AP) by Proteintech is a Polyclonal antibody targeting FCGR3B/CD16b in WB, IF/ICC, ELISA applications with reactivity to Human, rat samples 30201-1-AP targets FCGR3B/CD16b in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat samples. Tested Applications Positive WB detected in: Raji cells, U-937 cells, rat spleen tissue Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CD16 is a low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. In humans, FcγRIIIa/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues in the mature proteins(PMID: 30361439). FCGR3B/CD16b can be detected as 26 kDa (PMID: 34108956; 36096917) and 50-75 kDa (PMID: 30655272; 18208407). The antibody is specific for FCGR3B/CD16b. Specification Tested Reactivity: Human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: Fc fragment of IgG, low affinity IIIb, receptor (CD16b) Calculated Molecular Weight: 26 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: NM_000570 Gene Symbol: FCGR3B Gene ID (NCBI): 2215 RRID: AB_3086262 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75015 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.