Proteintech, 30244-1-AP, CXorf21 Polyclonal antibody

Size: 20ul / 150ul The CXorf21 (30244-1-AP) by Proteintech is a Polyclonal antibody targeting CXorf21 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 30244-1-AP targets CXorf21 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: THP-1 cells Positive IHC detected in: mouse skin tissue, human cervical cancer tissue, rat skin tissue, rat stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U-251 cells, U2OS cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CXorf21 (Chromosome X open reading frame 21) is also named as TASL and TLR adapter interacting with SLC15A4 on the lysosome. CXorf21 is an innate immune adapter that mediates the recruitment and activation of IRF5 downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9 (PMID:32433612). CXorf21 plays a role in the regulation of endolysosomal pH in immune cells such as B-cells, dendritic cells and monocytes (PMID: 31001245). CXorf21 is a mediator of the X-chromosome gene dose-dependent increased risk of SLE in females (PMID: 31695690). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag32773 Product name: Recombinant human CXorf21 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC020611 Sequence: MLSEGYLSGLEYWNDIHWSCASYNEQVAGEKEEETNSVATLSYSSVDETQVRSLYVSCKSSGKFISSVHSRESQHSRSQRVTVLQTNPNPVFESPNLAAVEICRDASRETYLVPSSCKSICKNYNDLQIAGGQVMAINSVTTDFPSESSFEYGPLLKSSEIPLPMEDSISTQPSDFPQKPIQRYSSYWRITSIKEKSSLQMQNPISNAVLNEYLEQKVVELYKQYIMDTVFHDSSPTQILASELIMTSVDQISLQVSREKNLETSKARDIVFSRLLQLMSTEITEISTPSLHISQYSNVNP Predict reactive species Full Name: chromosome X open reading frame 21 Observed Molecular Weight: 34 kDa GenBank Accession Number: BC020611 Gene Symbol: CXorf21 Gene ID (NCBI): 80231 RRID: AB_3086276 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9HAI6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.