Proteintech, 30324-1-AP, ICAM-1/CD54 Polyclonal antibody

Size: 20ul / 150ul The ICAM-1/CD54 (30324-1-AP) by Proteintech is a Polyclonal antibody targeting ICAM-1/CD54 in WB, IHC, IF-P, ELISA applications with reactivity to mouse, rat samples 30324-1-AP targets ICAM-1/CD54 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples. Tested Applications Positive WB detected in: mouse heart tissue, rat liver tissue, mouse liver tissue, mouse spleen tissue Positive IHC detected in: mouse liver tissue, mouse lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse liver tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information ICAM-1 (Intercellular Adhesion Molecule-1, CD54) is a glycosylated, transmembrane protein, a member of Ig super family. This protein involves in cell adhesion by interacting with a number of 2 integrin family members such as LFA-1 and Mac-1. Its expression is regulated by chemokines. This antibody is raised against mouse Icam-1. Specification Tested Reactivity: mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: intercellular adhesion molecule 1 Calculated Molecular Weight: 59 kDa Observed Molecular Weight: 95-110 kDa GenBank Accession Number: NM_010493 Gene Symbol: ICAM-1 Gene ID (NCBI): 15894 RRID: AB_3669710 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P13597 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.