Proteintech, 30412-1-AP, Haptoglobin Polyclonal antibody

Size: 20ul / 150ul The Haptoglobin (30412-1-AP) by Proteintech is a Polyclonal antibody targeting Haptoglobin in WB, IP, ELISA applications with reactivity to human, rat, pig, rabbit samples 30412-1-AP targets Haptoglobin in WB, IP, ELISA applications and shows reactivity with human, rat, pig, rabbit samples. Tested Applications Positive WB detected in: rabbit blood tissue, pig plasma, rabbit plasma Positive IP detected in: human plasma Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information Haptoglobin is also named as Zonulin and belongs to the peptidase S1 family. Haptoglobin is an alpha-2-glycoprotein synthesized mainly by hepatocytes consisting of both alpha and beta subunits linked by disulfide bonds forming dimers (PMID: 4018023). Haptoglobin is primarily produced in the liver, and when red cells are lysed in vivo, free hemoglobin binds to circulating haptoglobin; the hemoglobin-haptoglobin complex is then degraded by the reticuloendothelial system (PMID: 24809098). Because haptoglobin levels become depleted in the presence of large amounts of free hemoglobin, decreased haptoglobin is a marker of hemolysis. The human haptoglobin (HP) gene exists in two major allelic forms leading to three major genotypes: Hp1-1, Hp2-1, and Hp-2-2, that separate on alkaline starch gel electrophoresis due to different molecular weights (PMID: 17474882). Their properties vary in binding strength, anti-oxidative ability, and rate of clearance. Binding strength is greatest with Hp1-1, but the Hp-2 genotypes can bind a larger number of hemoglobin alpha-beta dimers. Complexes of Hp2-2 and hemoglobin have higher affinity for the CD163 receptor, the monocyte-macrophage receptor that facilitates its clearance. Neutrophils contained a large amount of highly glycosylated Hp (β-chain 45-65 kDa) synthesized in neutrophil precursors and stored in specific granules and a small amount of Hp (β-chain 39 kDa) endocytosed from plasma and stored in secretory vesicles (PMID: 16543473). Specification Tested Reactivity: human, rat, pig, rabbit Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: haptoglobin Observed Molecular Weight: 36-45 kDa GenBank Accession Number: BC121125 Gene Symbol: HP Gene ID (NCBI): 3240 RRID: AB_3669719 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P00738 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.