Proteintech, 30508-1-AP, ARHGEF11 Polyclonal antibody

Size: 20ul / 150ul The ARHGEF11 (30508-1-AP) by Proteintech is a Polyclonal antibody targeting ARHGEF11 in WB, IHC, ELISA applications with reactivity to human, mouse samples 30508-1-AP targets ARHGEF11 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293T cells, HuH-7 cells, MDA-MB-453 cells, mouse brain tissue Positive IHC detected in: mouse colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information ARHGEF11 (Rho guanine nucleotide exchange factor 11) is a specific guanine nucleotide exchange factor (GEF) for RhoA, which is a critical regulator of actin cytoskeleton dynamics and organization of dendritic spines and inhibitor of spine maintenance (PMID: 28036092). ARHGEF11 has been proven to promote tumor metastasis in glioblastoma and ovarian carcinoma (PMID: 33122451). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag33104 Product name: Recombinant human ARHGEF11 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1223-1522 aa of Sequence: ETQAAQEPEDDLTPTPSVISVTSHPWDPGSPGQAPPGGEGDNTQLAGLEGERPEQEDMGLCSLEHLPPRTRNSGIWESPELDRNLAEDASSTEAAGGYKVVRKAEVAGSKVVPALPESGQSEPGPPEVEGGTKATGNCFYVSMPSGPPDSSTDHSEAPMSPPQPDSLPAGQTEPQPQLQGGNDDPRRPSRSPPSLALRDVGMIFHTIEQLTLKLNRLKDMELAHRELLKSLGGESSGGTTPVGSFHTEAARWTDGSLSPPAKEPLASDSRNSHELGPCPEDGSDAPLEDSTADAAASPGP Predict reactive species Full Name: Rho guanine nucleotide exchange factor (GEF) 11 Calculated Molecular Weight: 168 kDa Observed Molecular Weight: 200-220 kDa Gene Symbol: ARHGEF11 Gene ID (NCBI): 9826 RRID: AB_3086341 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O15085 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.