Proteintech, 30592-1-AP, Mmp9 Polyclonal antibody

Size: 20ul / 150ul The Mmp9 (30592-1-AP) by Proteintech is a Polyclonal antibody targeting Mmp9 in WB, IF/ICC, IP, ELISA applications with reactivity to mouse, human samples 30592-1-AP targets Mmp9 in WB, IF/ICC, IP, ELISA applications and shows reactivity with mouse, human samples. Tested Applications Positive WB detected in: mouse lung tissue, mouse spleen tissue Positive IP detected in: mouse lung tissue Positive IF/ICC detected in: U2OS cells, A431 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Mmp9 (matrix metallopeptidase 9), also known as Clg4b. It is expected to be located inextracellular matrix. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The calculated molecular weight of Mmp9 is 80 kDa, the pro-MMP9 is 92 kDa, and this protein can exist as a dimer of 180 kDa (PMID:7492685). Specification Tested Reactivity: mouse, human Cited Reactivity: human, mouse, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: matrix metallopeptidase 9 Calculated Molecular Weight: 81kd Observed Molecular Weight: 80-100 kDa GenBank Accession Number: NM_013599 Gene Symbol: Mmp9 Gene ID (NCBI): 17395 RRID: AB_3086370 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P41245-1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.