Proteintech, 30601-1-AP, Fas/CD95 Polyclonal antibody

Size: 20ul / 150ul The Fas/CD95 (30601-1-AP) by Proteintech is a Polyclonal antibody targeting Fas/CD95 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 30601-1-AP targets Fas/CD95 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, J774A.1 cells, mouse brain tissue, rat brain tissue Positive IF/ICC detected in: Raji cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag30426 Product name: Recombinant mouse Fas/CD95 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 22-169 aa of NM_007987 Sequence: QGTNSISESLKLRRRVRETDKNCSEGLYQGGPFCCQPCQPGKKKVEDCKMNGGTPTCAPCTEGKEYMDKNHYADKCRRCTLCDEEHGLEVETNCTLTQNTKCKCKPDFYCDSPGCEHCVRCASCEHGTLEPCTATSNTNCRKQSPRNR Predict reactive species Full Name: Fas (TNF receptor superfamily member 6) Calculated Molecular Weight: 37 kDa Observed Molecular Weight: 30-40 kDa GenBank Accession Number: NM_007987 Gene Symbol: Fas/CD95 Gene ID (NCBI): 14102 RRID: AB_3086371 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P25446 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.