Proteintech, 30641-1-AP, ASC/TMS1 Polyclonal antibody

Size: 20ul / 150ul The ASC/TMS1 (30641-1-AP) by Proteintech is a Polyclonal antibody targeting ASC/TMS1 in WB, IHC, IF/ICC, ELISA applications with reactivity to mouse, rat samples 30641-1-AP targets ASC/TMS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples. Tested Applications Positive WB detected in: mouse spleen tissue, mouse thymus tissue, rat spleen tissue, rat thymus tissue Positive IHC detected in: mouse colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PYCARD (PYD and CARD domain containing), also known as ASC and CARD5. The calculated molecular weight of PYCARD is 21 kDa. It is located in cytosol, extracellular region and nucleus. PYCARD is also expressed in several structures, including alimentary system; brain; heart; hemolymphoid system gland; and urinary system. The gene enables identical protein binding activity and protein dimerization activity. Involved in several processes, including activation of cysteine-type endopeptidase activity; positive regulation of cytokine production; and regulation of defense response. It acts upstream of or within several processes, including defense response to Gram-positive bacterium; positive regulation of macromolecule metabolic process; and regulation of autophagy. It is also a part of inflammasome complex. Finally, it orthologous to human PYCARD (PYD and CARD domain containing). Specification Tested Reactivity: mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag32519 Product name: Recombinant mouse PYCARD protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-193 aa of NM_023258 Sequence: MGRARDAILDALENLSGDELKKFKMKLLTVQLREGYGRIPRGALLQMDAIDLTDKLVSYYLESYGLELTMTVLRDMGLQELAEQLQTTKEESGAVAAAASVPAQSTARTGHFVDQHRQALIARVTEVDGVLDALHGSVLTEGQYQAVRAETTSQDKMRKLFSFVPSWNLTCKDSLLQALKEIHPYLVMDLEQS Predict reactive species Full Name: PYD and CARD domain containing Calculated Molecular Weight: 21KD Observed Molecular Weight: 21-25 kDa GenBank Accession Number: NM_023258 Gene Symbol: Pycard Gene ID (NCBI): 66824 RRID: AB_3086379 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9EPB4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.