Proteintech, 30888-1-AP, Phospho-POLR2A (Ser2) Polyclonal antibody

Size: 20ul / 100ul The Phospho-POLR2A (Ser2) (30888-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-POLR2A (Ser2) in WB, ELISA applications with reactivity to Human, mouse samples 30888-1-AP targets Phospho-POLR2A (Ser2) in WB, ELISA applications and shows reactivity with Human, mouse samples. Tested Applications Positive WB detected in: C2C12 cells, λ phosphatase treated C2C12 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Background Information DNA-directed RNA polymerase II subunit RPB1 is the catalytic core component of DNA-dependent RNA polymerase II (Pol II). So that the information encoded in DNA can be used, RNA polymerase II makes copies of specific genes. To ensure that RNA polymerase II copies the correct genes at the correct time, a group of regulatory proteins are needed to control its activity. Many of these proteins interact with RNA polymerase II at a region known as the C-terminal domain, or CTD for short. For example, before RNA polymerase can make a full copy of a gene, a small molecule called a phosphate group must first be added to CTD at specific units known as Ser2. (PMID: 31385803) Specification Tested Reactivity: Human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa Calculated Molecular Weight: 1970 aa, 217 kDa Observed Molecular Weight: 250 kDa GenBank Accession Number: BC137231 Gene Symbol: POLR2A Gene ID (NCBI): 5430 RRID: AB_3669776 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P24928 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.