Proteintech, 30937-1-AP, Phospho-Cardiac Troponin I (Ser23/24) Polyclonal antibody

Size: 20ul / 100ul The Phospho-Cardiac Troponin I (Ser23/24) (30937-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-Cardiac Troponin I (Ser23/24) in WB, ELISA applications with reactivity to Human, mouse samples 30937-1-AP targets Phospho-Cardiac Troponin I (Ser23/24) in WB, ELISA applications and shows reactivity with Human, mouse samples. Tested Applications Positive WB detected in: mouse heart tissue, λ phosphatase treated mouse heart tissue Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information Cardiac Troponin I (cTnI) is produced by cardiac muscle. To date, most investigative effort in phosphorylation mediated regulation of cTnI function has focused on the actions of PKA and PKC. Evidence from studies in a variety of systems, ranging from reconstituted myofilament proteins to cultured myocytes or transgenic animals with heterologous expression of modified cTnI proteins, indicates that PKA-mediated functional effects (eg, decreased myofilament Ca2 sensitivity) are mediated principally through the phosphorylation of Ser22/23 (equivalent to Ser23/24, including the initiating Met, in the rodent sequence) within the unique N-terminal extension of cTnI. (PMID: 15514163) Specification Tested Reactivity: Human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: troponin I type 3 (cardiac) Calculated Molecular Weight: 210 aa, 24 kDa Observed Molecular Weight: 26 kDa GenBank Accession Number: BC096165 Gene Symbol: Cardiac Troponin I Gene ID (NCBI): 7137 RRID: AB_3669787 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P19429 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.