Proteintech, 31206-1-AP, ASCL1 Polyclonal antibody

Size: 20ul / 150ul The ASCL1 (31206-1-AP) by Proteintech is a Polyclonal antibody targeting ASCL1 in WB, IHC, ELISA applications with reactivity to human samples 31206-1-AP targets ASCL1 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: K-562 cells, NCI-H1299 cells, U-251 cells, PC-3 cells Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:400-1:1600 Background Information ASCL1 (achaete-scute family bHLH transcription factor 1), also known as ASH1. It is expected to be located in nucleus. Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach. The calculated molecular weight of ASCL1 is 25 kDa. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag35185 Product name: Recombinant human ASCL1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-118 aa of BC003134 Sequence: MESSAKMESGGAGQQPQPQPQQPFLPPAACFFATAAAAAAAAAAAAAQSAQQQQQQQQQQQQAPQLRPAADGQPSGGGHKSAPKQVKRQRSSSPELMRCKRRLNFSGFGYSLPQQQPA Predict reactive species Full Name: achaete-scute complex homolog 1 (Drosophila) Calculated Molecular Weight: 25 kDa Observed Molecular Weight: 25 kDa GenBank Accession Number: BC003134 Gene Symbol: ASCL1 Gene ID (NCBI): 429 RRID: AB_3669901 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: P50553 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.