Proteintech, 31247-1-AP, Lif Polyclonal antibody

Size: 20ul / 150ul The Lif (31247-1-AP) by Proteintech is a Polyclonal antibody targeting Lif in WB, ELISA applications with reactivity to mouse samples 31247-1-AP targets Lif in WB, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: NIH/3T3 cells, mouse lung tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF is a glycoprotein containing six putative N-glycosylation sites. The molecular weight of the unglycosylated LIF protein is 20-25 kDa, while the molecular weight of the glycosylated protein is in the range of 37-63 kDa. Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: leukemia inhibitory factor Calculated Molecular Weight: 22 KDa Observed Molecular Weight: 33-35 kDa Gene Symbol: Lif Gene ID (NCBI): 16878 RRID: AB_3669916 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P09056 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.