Proteintech, 31539-1-AP, COG4 Polyclonal antibody

Size: 20ul / 150ul The COG4 (31539-1-AP) by Proteintech is a Polyclonal antibody targeting COG4 in WB, IHC, ELISA applications with reactivity to human, mouse samples 31539-1-AP targets COG4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HT-1080 cells, mouse spermatophore tissue, MRC-5 cells, hTERT-RPE1 cells, mouse testis tisuue Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information COG complex subunit 4 is present in high molecular weight complexes that govern membrane movement between the Golgi and ER. Cog4 dependent trafficking and secretion of ECM components are required for protrusion and extension of the epithelial projections of the inner ear (PMID: 30287385). Loss-of-function COG4-mutant cells have impaired glycosylation and reduced COG4 protein levels (PMID: 30290151). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag35295 Product name: Recombinant human COG4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 506-785 aa of NM_001365426.1 Sequence: FQDIQRGVTSAVNIMHSSLQQGKFDTKGIESTDEAKMSFLVTLNNVEVCSENISTLKKTLESDCTKLFSQGIGGEQAQAKFDSCLSDLAAVSNKFRDLLQEGLTELNSTAIKPQVQPWINSFFSVSHNIEEEEFNDYEANDPWVQQFILNLEQQMAEFKASLSPVIYDSLTGLMTSLVAVELEKVVLKSTFNRLGGLQFDKELRSLIAYLTTVTTWTIRDKFARLSQMATILNLERVTEILDYWGPNSGPLTWRLTPAEVRQVLALRIDFRSEDIKRLRL Predict reactive species Full Name: component of oligomeric golgi complex 4 Calculated Molecular Weight: 89kDa，785aa Observed Molecular Weight: 89 kDa GenBank Accession Number: NM_001365426.1 Gene Symbol: COG4 Gene ID (NCBI): 25839 RRID: AB_3670028 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q9H9E3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.