Proteintech, 31675-1-AP, CCDC102B Polyclonal antibody

Size: 20ul / 150ul The CCDC102B (31675-1-AP) by Proteintech is a Polyclonal antibody targeting CCDC102B in IF/ICC, IP, ELISA applications with reactivity to human samples 31675-1-AP targets CCDC102B in IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive IP detected in: SH-SY5Y cells, MOLT-4 cells Positive IF/ICC detected in: A431 cells Recommended dilution Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Coiled coil domain containing 102B (CCDC102B) is a new centrosome-linker protein that is required for maintaining centrosome cohesion. Human CCDC102B is an uncharacterized protein containing 513 amino acids (aa); it is predicted to contain one short coiled-coil domain on its N-terminus and two long coiled-coil domains in the middle region and C-terminus, respectively (PMID: 35957886). At the onset of mitosis, along with other centrosome components, CCDC102B is phosphorylated by Nek2A and is disassociated from the centrosome to allow for the centrosome separation (PMID: 30404835). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag36379 Product name: Recombinant human CCDC102B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-240 aa of BC126448 Sequence: MKPNLDGVDLFNNGGSGNGETKTGLRLKAINLPLENEVTEISALQVHLDEFQKILWKEREMRTALEKEIERLESALSLWKWKYEELKESKPKNVKEFDILLGQHNDEMQELSGNIKEESKSQNSKDRVICELRAELERLQAENTSEWDKREILEREKQGLERENRRLKIQVKEMEELLDKKNRLSANSQSPDFKMSQIDLQEKNQELLNLQHAYYKLNRQYQANIAELTHANNRVDQNEA Predict reactive species Full Name: coiled-coil domain containing 102B Observed Molecular Weight: 60~68 kDa GenBank Accession Number: BC126448 Gene Symbol: CCDC102B Gene ID (NCBI): 79839 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q68D86 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.