Proteintech, 31732-1-AP, STAP1 Polyclonal antibody

Size: 20ul / 150ul The STAP1 (31732-1-AP) by Proteintech is a Polyclonal antibody targeting STAP1 in WB, IF/ICC, ELISA applications with reactivity to human samples 31732-1-AP targets STAP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Raji cells, Ramos cells Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Signal transducing adaptor protein 1 (STAP1), also known as BRDG1, contains a proline-rich region, a pleckstrin homology (PH) domain, and a region in the carboxy terminal half with similarity to the Src Homology 2 (SH2) domain. STAP1 acts downstream of the Tec tyrosine kinase and participates in a positive feedback loop by increasing the activity of Tec (PMID: 10518561). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag35196 Product name: Recombinant human STAP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 139-293 aa of BC014958 Sequence: HEVLEREKKRRIETEQSTSVEKEKEPTEDYVDVLNPMPACFYTVSRKEATEMLQKNPSLGNMILRPGSDSRNYSITIRQEIDIPRIKHYKVMSVGQNYTIELEKPVTLPNLFSVIDYFVKETRGNLRPFICSTDENTGQEPSMEGRSEKLKKNPH Predict reactive species Full Name: signal transducing adaptor family member 1 Calculated Molecular Weight: 34 kDa Observed Molecular Weight: 34 kDa GenBank Accession Number: BC014958 Gene Symbol: STAP1 Gene ID (NCBI): 26228 RRID: AB_3670102 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q9ULZ2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.