Proteintech, 31921-1-AP, C7orf27 Polyclonal antibody

Size: 20ul / 150ul The C7orf27 (31921-1-AP) by Proteintech is a Polyclonal antibody targeting C7orf27 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 31921-1-AP targets C7orf27 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A431 cells, HEK-293 cells, Hela cells, Jurkat cells, U2OS cells Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:400-1:1600 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information C7orf27, also known as BRAT1, not only plays a role in DNA damage response, but also participates in the regulation of cell growth and apoptosis, which further regulates mitochondrial metabolism and tumorigenesis. Moreover, several studies have shown that BRAT1 mutations are associated with neurodevelopmental and neurodegenerative diseases. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag37056 Product name: Recombinant human C7orf27 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 600-821 aa of BC015632 Sequence: LSVDSEGFPRRAVMQVFTEWLRDGHADAAQDTEQFVATVLQAASRDLDWEVRAQGLELALVFLGQTLGPPRTHCPYAVALPEVAPAQPLTEALRALCHVGLFDFAFCALFDCDRPVAQKSCDLLLFLRDKIASYSSLREARGSPNTASAEATLPRWRAGEQAQPPGDQEPEAVLAMLRSLDLEGLRSTLAESSDHVEKSPQSLLQDMLATGGFLQGDEADCY Predict reactive species Full Name: chromosome 7 open reading frame 27 Observed Molecular Weight: 75 kDa GenBank Accession Number: BC015632 Gene Symbol: C7orf27 Gene ID (NCBI): 221927 RRID: AB_3670145 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q6PJG6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.