Proteintech, 31950-1-AP, ICAM2/CD102 Polyclonal antibody

Size: 20ul / 150ul The ICAM2/CD102 (31950-1-AP) by Proteintech is a Polyclonal antibody targeting ICAM2/CD102 in WB, IHC, IP, ELISA applications with reactivity to mouse samples 31950-1-AP targets ICAM2/CD102 in WB, IHC, IP, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: mouse lung tissue, rat lung tissue Positive IP detected in: bEnd.3 cells Positive IHC detected in: mouse spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information Intercellular adhesion molecule 2 (ICAM2) is also named CD102. ICAM2 is a type I transmembrane glycoprotein containing two immunoglobulin-like domains, which are crucial to lymphocyte recirculation and mediate cell survival signals via pathways associated with LFA-1 (PMID: 2497351, PMID: 11825565). ICAM2 has been reported to be a mediator for a survival signal sufficient to block apoptosis by activation of the PI3K/AKT pathway (PMID: 11825565). ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187. Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Eg1540 Product name: Recombinant Mouse ICAM-2/CD102 protein (His Tag) Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 23-222 aa of NM_010494.2 Sequence: KAFEVYIWSEKQIVEATESWKINCSTNCAAPDMGGLETPTNKIMLEEHPQGKWKQFLVSNVSKDTVFFCHFTCSGKQHSESLNIRVYQPPAQVTLKLQPPRVFVGEDFTIECTVSPVQPLERLTLSLLRGRETLKNQTFGGAETVPQEATATFNSTALKKDGLNFSCQAELDLRPHGGYIIRSISEYQILEVYEPMQDNQ Predict reactive species Full Name: intercellular adhesion molecule 2 Calculated Molecular Weight: 31kDa Observed Molecular Weight: 45-55 kDa GenBank Accession Number: NM_010494.2 Gene Symbol: Icam2 Gene ID (NCBI): 15896 RRID: AB_3670156 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: P35330 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.