Proteintech, 31998-1-AP, CD6 Polyclonal antibody

Size: 20ul / 150ul The CD6 (31998-1-AP) by Proteintech is a Polyclonal antibody targeting CD6 in WB, IHC, IF-P, ELISA applications with reactivity to mouse, rat samples 31998-1-AP targets CD6 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples. Tested Applications Positive WB detected in: rat spleen tissue, rat thymus tissue Positive IHC detected in: mouse thymus tissue, rat thymus tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse spleen tissue Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information CD6 is a type I transmembrane glycoprotein that belongs to the scavenger receptor cysteine-rich superfamily (PMID: 21880988). It is composed of an extracellular region, which consists of three tandem SRCR domains, a transmembrane region, and a cytoplasmic tail devoid of intrinsic catalytic activity but harboring several phosphorylatable residues suitable for intracellular signal transduction (PMID: 38139340; 23711376). CD6 is expressed by T cells, medullary thymocytes, a subset of B cells and NK cells, and in some cells of the brain (PMID: 1919444; 21178331). CD6 plays a role in lymphocyte activation, proliferation, and survival processes via interaction with its endogenous ligands. Specification Tested Reactivity: mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Eg1401 Product name: Recombinant Mouse CD6 protein (rFc Tag) Source: mammalian cells -derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 17-396 aa of NM_009852.3 Sequence: GLPSPAPSGQHKNGTIPNMTLDLEERLGIRLVNGSSRCSGSVKVLLESWEPVCAAHWNRAATEAVCKALNCGDSGKVTYLMPPTSELPPGATSGNTSSAGNTTWARAPTERCRGANWQFCKVQDQECSSDRRLVWVTCAENQAVRLVDGSSRCAGRVEMLEHGEWGTVCDDTWDLQDAHVVCKQLKCGWAVKALAGLHFTPGQGPIHRDQVNCSGTEAYLWDCPGRPGDQYCGHKEDAGVVCSEHQSWRLTGGIDSCEGQVEVYFRGVWSTVCDSEWYPSEAKVLCRSLGCGSAVARPRGLPHSLDGRMYYSCKGQEPALSTCSWRFNNSNLCSQSRAARVVCSGSQRHLNLSTSEVPSRVPVTIESSVPVSVKDKDSQG Predict reactive species Full Name: CD6 antigen Calculated Molecular Weight: 72kDa Observed Molecular Weight: 100-130 kDa GenBank Accession Number: NM_009852.3 Gene Symbol: Cd6 Gene ID (NCBI): 12511 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q61003-1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.