Proteintech, 32034-1-AP, FAM162A Polyclonal antibody

Size: 20ul / 150ul The FAM162A (32034-1-AP) by Proteintech is a Polyclonal antibody targeting FAM162A in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 32034-1-AP targets FAM162A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Caki-2 cells, HepG2 cells, RT-4 cells Positive IHC detected in: mouse colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Family with sequence similarity 162 member A (FAM162A, also known as E2IG5, HGTD-P, and C3orf28) is a mitochondrial apoptotic protein, which is known as an HIF-1 alpha-responsive pro-apoptotic molecule (PMID: 15082785; 16698020; 22776087). When FAM162A was overexpressed, the hypoxia signal was sent directly to the mitochondria and caused cell death (PMID: 16698020). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag36569 Product name: Recombinant human FAM162A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-154 aa of BC015060 Sequence: MGSLSGLRLAAGSCFRLCERDVSSSLRLTRSSDLKRINGFCTKPQESPGVPSRTYNRVPLHKPTDWQKKILIWSGRFKKEDEIPETVSLEMLDAAKNKMRVKISYLMIALTVVGCIFMVIEGKKAAQRHETLTSLNLEKKARLKEEAAMKAKTE Predict reactive species Full Name: family with sequence similarity 162, member A Calculated Molecular Weight: 154 aa, 17 kDa Observed Molecular Weight: 17 kDa GenBank Accession Number: BC015060 Gene Symbol: FAM162A Gene ID (NCBI): 26355 RRID: AB_3670175 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q96A26 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.