Proteintech, 32051-1-AP, Factor IX/PTC Polyclonal antibody

Size: 20ul / 150ul The Factor IX/PTC (32051-1-AP) by Proteintech is a Polyclonal antibody targeting Factor IX/PTC in WB, IHC, ELISA applications with reactivity to human, mouse samples 32051-1-AP targets Factor IX/PTC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, HepG2 cells, L02 cells Positive IHC detected in: mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information The F9 gene encodes coagulation factor IX, which circulates as an inactive zymogen until the proteolytic release of its activation peptide allows it to assume the conformation of an active serine protease. Its role in the blood coagulation cascade is to activate factor X (F10) through interactions with calcium, membrane phospholipids, and factor VIII (F8). Factor IX and factor X both consist of 2 polypeptide chains referred to as the L (light) and H (heavy) chains. The H chain bears a structural resemblance to the polypeptide chain of the pancreatic serine protease trypsin (PRSS1). The L chain is covalently linked to the H chain by a single disulfide bond. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Eg1045 Product name: Recombinant Human Coagulation factor IX protein (His Tag) Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 29-461 aa of NM_000133.4 Sequence: TVFLDHENANKILNRPKRYNSGKLEEFVQGNLERECMEEKCSFEEAREVFENTERTTEFWKQYVDGDQCESNPCLNGGSCKDDINSYECWCPFGFEGKNCELDVTCNIKNGRCEQFCKNSADNKVVCSCTEGYRLAENQKSCEPAVPFPCGRVSVSQTSKLTRAETVFPDVDYVNSTEAETILDNITQSTQSFNDFTRVVGGEDAKPGQFPWQVVLNGKVDAFCGGSIVNEKWIVTAAHCVETGVKITVVAGEHNIEETEHTEQKRNVIRIIPHHNYNAAINKYNHDIALLELDEPLVLNSYVTPICIADKEYTNIFLKFGSGYVSGWGRVFHKGRSALVLQYLRVPLVDRATCLRSTKFTIYNNMFCAGFHEGGRDSCQGDSGGPHVTEVEGTSFLTGIISWGEECAMKGKYGIYTKVSRYVNWIKEKTKLT Predict reactive species Full Name: coagulation factor IX Calculated Molecular Weight: 52kDa Observed Molecular Weight: 30-33 kDa, 50 kDa GenBank Accession Number: NM_000133.4 Gene Symbol: F9 Gene ID (NCBI): 2158 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: P00740-1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.