Proteintech, 32141-1-AP, CD300c Polyclonal antibody

Size: 20ul / 150ul The CD300c (32141-1-AP) by Proteintech is a Polyclonal antibody targeting CD300c in IHC, ELISA applications with reactivity to human samples 32141-1-AP targets CD300c in IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information CD300c is a member of the CD300 family of receptors, which are type I transmembrane proteins expressed in both myeloid and lymphoid lineages and modulate immune responses by their stimulatory and inhibitory capabilities (PMID: 30485414). CD300c, also known as CLM-6, CMRF-35 and IgSF16, is composed of an IgV-like extracellular domain, a transmembrane domain and a short cytoplasmic tail. It is expressed on the surface of monocytes, macrophages, dendritic cells, neutrophils, some T and B lymphocytes (PMID: 1349532; 30498497). CD300c has been reported as an activating receptor expressed on monocytes and that it has a potential role in inflammatory responses (PMID: 23571507). It has also been identified as a T cell co-inhibitory molecule (PMID: 30498497). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: CD300c molecule Calculated Molecular Weight: 224 aa, 25 kDa GenBank Accession Number: BC022279 Gene Symbol: CD300C Gene ID (NCBI): 10871 RRID: AB_3670205 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q08708 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.