Proteintech, 32387-1-AP, CD31 Polyclonal antibody

Size: 20ul / 150ul The CD31 (32387-1-AP) by Proteintech is a Polyclonal antibody targeting CD31 in WB, IHC, ELISA applications with reactivity to mouse samples 32387-1-AP targets CD31 in WB, IHC, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: bEnd.3 cells Positive IHC detected in: mouse liver tissue, mouse brain tissue, mouse spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information CD31, also known as PECAM1 (Platelet endothelial cell adhesion molecule), is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. It is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, CD31 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489). Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: platelet/endothelial cell adhesion molecule 1 Calculated Molecular Weight: 81 kDa Observed Molecular Weight: 120 kDa GenBank Accession Number: NM_001305157.1 Gene Symbol: CD31 Gene ID (NCBI): 18613 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q08481-1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.