Proteintech, 32456-1-AP, PRAS40 Polyclonal antibody

Size: 20ul / 150ul The PRAS40 (32456-1-AP) by Proteintech is a Polyclonal antibody targeting PRAS40 in WB, IF/ICC, IP, ELISA applications with reactivity to human samples 32456-1-AP targets PRAS40 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, Calu-1 cells, HeLa cells, HepG2 cells, L02 cells Positive IP detected in: HeLa cells Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information PRAS40 (40 kDa proline-rich AKT substrate) is also named as AKT1S1 (Proline-rich AKT1 substrate 1). PRAS40 is a substrate of Akt and a component of the mammalian target of rapamycin complex 1 (mTORC1) (PMID: 28978182). Locating at the crossroad of the PI3K/Akt pathway and the mTOR pathway, PRAS40 is phosphorylated by growth factors or other stimuli, and regulates the activation of these signaling pathways in turn (PMID: 28978182). PRAS40 is expressed in a variety of tissues in vivo and has multiple phosphorylation sites, which its activity is closely related to phosphorylation. Studies have shown that PRAS40 is involved in regulating cell growth, cell apoptosis, oxidative stress, autophagy and angiogenesis, as well as various of signalling pathways such as mammalian target of mammalian target rapamycin (mTOR), protein kinase B (PKB/Akt), nuclear factor kappa-B(NF-κB) proto-oncogene serine/threonine-protein kinase PIM-1(PIM1) and pyruvate kinase M2 (PKM2) (PMID: 33504218). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag35446 Product name: Recombinant human PRAS40 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 131-256 aa of BC051844 Sequence: MDEDATLQDLPPFCESDPESTDDGSLSEETPAGPPTCSVPPASALPTQQYAKSLPVSVPVWGFKEKRTEARSSDEENGPPSSPDLDRIAASMRALVLREAEDTQVFGDLPRPRLNTSDFQKLKRKY Predict reactive species Full Name: AKT1 substrate 1 (proline-rich) Calculated Molecular Weight: 27 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC051844 Gene Symbol: PRAS40 Gene ID (NCBI): 84335 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q96B36 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.