Proteintech, 32534-1-AP, Siglec-12 Polyclonal antibody

Size: 20ul / 150ul The Siglec-12 (32534-1-AP) by Proteintech is a Polyclonal antibody targeting Siglec-12 in WB, IHC, ELISA applications with reactivity to mouse, rat samples 32534-1-AP targets Siglec-12 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples. Tested Applications Positive WB detected in: mouse spleen tissue, rat spleen tissue Positive IHC detected in: mouse spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily that contain sialoside-binding N-terminal domains (PMID: 22749793). Sialic acid binding Ig-like lectin 12 (Siglec-12, also known as S2V, SLG, SIGLECL1, and Siglec-XII) is a member of the SIGLEC3-like subfamily of SIGLECs, which is highly expressed in the spleen, such as CD8+ cells, macrophages, neutrophils, and dendritic cells. It mediates sialic-acid-dependent binding to cells and could be an identification of therapeutic targets and prognostic biomarkers of sarcomas (PMID: 38182638). Specification Tested Reactivity: mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Eg2733 Product name: Recombinant Mouse Siglec-12 protein (rFc Tag) Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 19-350 aa of NM_031181.2 Sequence: QNPQEVFTLNVERKVVVQEGLCVLVPCNFSYLKKRLTDWTDSDPVHGFWYREGTDRRKDSIVATNNPIRKAVKETRNRFFLLGDPWRNDCSLNIREIRKKDAGLYFFRLERGKTKYNYMWDKMTLVVTALTNTPQILLPETLEAGHPSNLTCSVPWDCGWTAPPIFSWTGTSVSFLSTNTTGSSVLTITPQPQDHGTNLTCQVTLPGTNVSTRMTIRLNVSYAPKNLTVTIYQGADSVSTILKNGSSLPISEGQSLRLICSTDSYPPANLSWSWDNLTLCPSKLSKPGLLELFPVHLKHGGVYTCQAQHALGSQHISLSLSPQSSATLSEMM Predict reactive species Full Name: sialic acid binding Ig-like lectin E Calculated Molecular Weight: 52 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: NM_031181.2 Gene Symbol: Siglec-E Gene ID (NCBI): 83382 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q91Y57 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.