Proteintech, 32709-1-AP, DjC8 Polyclonal antibody

Size: 20ul / 150ul The DjC8 (32709-1-AP) by Proteintech is a Polyclonal antibody targeting DjC8 in WB, ELISA applications with reactivity to human samples 32709-1-AP targets DjC8 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: COLO 320 cells, Caco-2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information DjC8 (C8, a member of DNAJ heat shock protein family), also known as DNAJC8, which is a highly conserved protein, belonging to heat shock protein 40(HSP40) family. The protein is expressed in many cell types, including central nervous system macrophages, cerebellar inhibitory neurons, choroid plexus epithelial cells of ventricles, oligodendrocyte precursor cells and so on. In normal tissues, the expression level of DjC8 is relatively low, but it is high in some tissues such as testis, skeletal muscle and spleen. In hepatocellular carcinoma, the high expression of DjC8 is related to poor prognosis. Knocking down DjC8 can inhibit the proliferation, migration and invasion of hepatocellular carcinoma cells, and reduce the IC50 value of sorafenib, indicating that it may be a potential therapeutic target and prognostic marker for hepatocellular carcinoma. In addition, DjC8 is regulated by TIG1 in cervical cancer and participates in glycolysis. DjC8 contains a conserved J domain, which can interact with HSP70 and activate its ATPase activity, thus participating in the folding, unfolding, transport and degradation of protein. The molecular weight of DjC8 is 30 kDa. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag38520 Product name: Recombinant human DNAJC8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-253 aa of NM_014280.2 Sequence: MAASGESGTSGGGGSTEEAFMTFYSEVKQIEKRDSVLTSKNQIERLTRPGSSYFNLNPFEVLQIDPEVTDEEIKKRFRQLSILVHPDKNQDDADRAQKAFEAVDKAYKLLLDQEQKKRALDVIQAGKEYVEHTVKERKKQLKKEGKPTIVEEDDPELFKQAVYKQTMKLFAELEIKRKEREAKEMHERKRQREEEIEAQEKAKREREWQKNFEESRDGRVDSWRNFQANTKGKKEKKNRTFLRPPKVKMEQRE Predict reactive species Full Name: DnaJ (Hsp40) homolog, subfamily C, member 8 Calculated Molecular Weight: 30kDa，253aa Observed Molecular Weight: 30 kDa GenBank Accession Number: NM_014280.2 Gene Symbol: DNAJC8 Gene ID (NCBI): 22826 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: O75937 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.