Proteintech, 32824-1-AP, Granzyme B Polyclonal antibody

Size: 20ul / 150ul The Granzyme B (32824-1-AP) by Proteintech is a Polyclonal antibody targeting Granzyme B in WB, ELISA applications with reactivity to mouse samples 32824-1-AP targets Granzyme B in WB, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: CTLL-2 cells Recommended dilution Western Blot (WB): WB : 1:20000-1:100000 Background Information GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Eg3313 Product name: recombinant mouse Granzyme B protein Source: mammalian cells -derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 19-247 aa of NM_013542.3 Sequence: GEIIGGHEVKPHSRPYMALLSIKDQQPEAICGGFLIREDFVLTAAHCEGSIINVTLGAHNIKEQEKTQQVIPMVKCIPHPDYNPKTFSNDIMLLKLKSKAKRTRAVRPLNLPRRNVNVKPGDVCYVAGWGRMAPMGKYSNTLQEVELTVQKDRECESYFKNRYNKTNQICAGDPKTKRASFRGDSGGPLVCKKVAAGIVSYGYKDGSPPRAFTKVSSFLSWIKKTMKSS Predict reactive species Full Name: granzyme B Calculated Molecular Weight: 27KD Observed Molecular Weight: 27-30 kDa GenBank Accession Number: NM_013542.3 Gene Symbol: Gzmb Gene ID (NCBI): 14939 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: P04187 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.