Proteintech, 32874-1-AP, TXNDC15 Polyclonal antibody

Size: 20ul / 150ul The TXNDC15 (32874-1-AP) by Proteintech is a Polyclonal antibody targeting TXNDC15 in WB, IHC, ELISA applications with reactivity to human samples 32874-1-AP targets TXNDC15 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, MCF-7 cells, RKO cells, fetal human brain tissue Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:300-1:1200 Background Information TXNDC15 (thioredoxin domain-containing protein 15) is a single-pass type I transmembrane protein that contains a thioredoxin domain and is primarily localized in the cytoplasm. It plays a positive regulatory role in the Hedgehog signaling pathway within cilia and actively participates in the process of ciliogenesis, exerting significant influence on the structure and function of cilia-an essential cellular organelle. Additionally, TXNDC15 has been found to form mixed disulfide complexes with endoplasmic reticulum-resident protein disulfide isomerase (PDI) family members, such as ERp57, PDI, and ERp44, suggesting its involvement in protein folding and quality control processes. Notably, mutations in the TXNDC15 gene are associated with a rare and lethal genetic disorder known as Meckel-Gruber syndrome, which is caused by defects in ciliogenesis. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag37138 Product name: Recombinant human TXNDC15 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 33-321 aa of BC032568 Sequence: VEVAEESGRLWSEEQPAHPLQVGAVYLGEEELLHDPMGQDRAAEEANAVLGLDTQGDHMVMLSVIPGEAEDKVSSEPSGVTCGAGGAEDSRCNVRESLFSLDGAGAHFPDREEEYYTEPEVAESDAAPTEDSNNTESLKSPKVNCEERNITGLENFTLKILNMSQDLMDFLNPNGSDCTLVLFYTPWCRFSASLAPHFNSLPRAFPALHFLALDASQHSSLSTRFGTVAVPNILLFQGAKPMARFNHTDRTLETLKIFIFNQTGIEAKKNVVVTQADQIGPLPSTLIKS Predict reactive species Full Name: thioredoxin domain containing 15 Observed Molecular Weight: 55 kDa GenBank Accession Number: BC032568 Gene Symbol: TXNDC15 Gene ID (NCBI): 79770 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q96J42 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.