Proteintech, 32938-1-AP, TTBK1 Polyclonal antibody

Size: 20ul / 150ul The TTBK1 (32938-1-AP) by Proteintech is a Polyclonal antibody targeting TTBK1 in WB, ELISA applications with reactivity to human, mouse, rat samples 32938-1-AP targets TTBK1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: SH-SY5Y cells, fetal human brain tissue, mouse brain tissue, rat brain tissue Recommended dilution Western Blot (WB): WB : 1:6000-1:12000 Background Information TTBK1 (Tau Tubulin kinase 1), also known as BDTK (brain-derived Tau kinase), is a 1,321 amino acid protein that contains one protein kinase domain and belongs to the serine/threonine protein kinase family. Localized to the cytoplasm and expressed at high levels in brain and at lower levels in testis and spinal cord, TTBK1 functions as a serine/threonine kinase that can phosphorylate Tau (a protein involved in Tubulin polymerization) on threonine, tyrosine and serine residues. Specifically, TTBK1 uses divalent cations, such as magnesium and manganese, to catalyze the ATP-dependent transfer of a phosphate group onto Tau, creating a phosphoprotein and ADP. Phosphorylation of Tau causes its aggregation and subsequent loss of function, suggesting an important role for TTBK1 in the control of Tubulin dynamics. Two isoforms of TTBK1 are expressed due to alternative splicing events. The molecular weight observed is consistent with what has been described in PMID:16923168. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag36655 Product name: Recombinant human TTBK1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1060-1321 aa of BC036764 Sequence: LSEEDTGSEPSGSLSAKERWSKRARPQQDLARLVMEKRQGRLLLRLASGASSSSSEEQRRASETLSGTGSEEDTPASEPAAALPRKSGRAAATRSRIPRPIGLRMPMPVAAQQPASRSHGAAPALDTAITSRLQLQTPPGSATAADLRPKQPPGRGLGPGRAQAGARPPAPRSPRLPASTSAARNASASPRSQSLSRRESPSPSHQARPGVPPPRGVPPARAQPDGTPSPGGSKKGPRGKLQAQRATTKGRAGGAEGRAGAR Predict reactive species Full Name: tau tubulin kinase 1 Calculated Molecular Weight: 142/70 kDa Observed Molecular Weight: 200 kDa GenBank Accession Number: BC036764 Gene Symbol: TTBK1 Gene ID (NCBI): 84630 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: Q5TCY1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.