Proteintech, 33180-1-AP, Serpin E1/PAI-1 Polyclonal antibody

Size: 20ul / 150ul The Serpin E1/PAI-1 (33180-1-AP) by Proteintech is a Polyclonal antibody targeting Serpin E1/PAI-1 in WB, IF-P, ELISA applications with reactivity to human, rat samples 33180-1-AP targets Serpin E1/PAI-1 in WB, IF-P, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: U-251 cells, HuH-7 cells, U-87 MG cells Positive IF-P detected in: human placenta tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information Serpin E1/PAI-1 is a key member of the serine protease inhibitor (serpin) superfamily. It is the primary physiological inhibitor of the fibrinolytic system, negatively regulating fibrin degradation by rapidly inhibiting tissue-type plasminogen activator (tPA) and urokinase-type plasminogen activator (uPA). This central role enables PAI-1 to critically influence thrombus formation, tissue repair, and fibrosis. The expression of PAI-1 is induced by various signaling stimuli, and its elevated levels are closely associated with thrombotic diseases, metabolic syndrome, organ fibrosis, and cancer progression. Furthermore, PAI-1 serves as a marker of the senescence-associated secretory phenotype (SASP) and is involved in the process of cellular senescence. Therefore, PAI-1 is not only a core regulator of the balance between coagulation and fibrinolysis but also an important disease biomarker and therapeutic target. Specification Tested Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Eg3256 Product name: Recombinant Rat Serpin E1/PAI-1 protein (rFc Tag) Source: mammalian cells -derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 24-402 aa of NM_012620.1 Sequence: SPLPESHTAQQATNFGVKVFQHVVQASKDRNVVFSPYGVSSVLAMLQLTTAGKTRQQIQDAMGFNISERGTAPALRKLSKELMGSWNKNEISTADAIFVQRDLELVQGFMPHFFKLFRTTVKQVDFSEVERARFIINDWVERHTKGMISDLLAKGAVNELTRLVLVNALYFNGQWKTPFLEASTHQRLFHKSDGSTISVPMMAQNNKFNYTEFTTPDGHEYDILELPYHGETLSMFIAAPFEKDVPLSAITNILDAELIRQWKSNMTRLPRLLILPKFSLETEVDLRGPLEKLGMTDIFSSTQADFTSLSDQEQLSVAQALQKVKIEVNESGTVASSSTAILVSARMAPTEMVLDRSFLFVVRHNPTETILFMGQLMEP Predict reactive species Full Name: serine (or cysteine) peptidase inhibitor, clade E, member 1 Calculated Molecular Weight: 45 kDa Observed Molecular Weight: 45 kDa GenBank Accession Number: NM_012620.1 Gene Symbol: Serpine1 Gene ID (NCBI): 24617 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: P20961 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.