Proteintech, 33685-1-AP, YKT6 Polyclonal antibody

Size: 20ul / 150ul The YKT6 (33685-1-AP) by Proteintech is a Polyclonal antibody targeting YKT6 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, rat samples 33685-1-AP targets YKT6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: A549 cells, Calu-1 cells, HeLa cells, rat brain tissue Positive IHC detected in: human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells, U-251 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information YKT6 is a soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) that plays a central role in membrane fusion in eukaryotic cells. It is primarily localized in the cytoplasm and, through its unique dual lipidation modification (palmitoylation and farnesylation), can associate with various organelle membranes, such as the Golgi apparatus, endosomes, and vesicles. By forming stable trans-SNARE complexes with other SNARE proteins (e.g., Syntaxin and SNAP-25 family members) on target membranes, YKT6 provides the driving force for membrane fusion, thereby facilitating intracellular trafficking. This protein is essential for maintaining organelle integrity, autophagy, and secretory pathways. Studies have shown that dysfunction of YKT6 is associated with various diseases, including cancer, neurodegenerative disorders, and metabolic abnormalities, making it a potential target for drug development. Specification Tested Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag38740 Product name: Recombinant human YKT6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 111-198 aa of BC007319 Sequence: PVGSPATIHYPALDGHLSRYQNPREADPMTKVQAELDETKIILHNTMESLLERGEKLDDLVSKSEVLGTQSKAFYKTARKQNSCCAIM Predict reactive species Full Name: YKT6 v-SNARE homolog (S. cerevisiae) Calculated Molecular Weight: 22 kDa Observed Molecular Weight: 25 kDa GenBank Accession Number: BC007319 Gene Symbol: YKT6 Gene ID (NCBI): 10652 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: O15498 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.