Proteintech, 34112-1-AP, CSF1R/CD115 Polyclonal antibody

Size: 20ul / 150ul The CSF1R/CD115 (34112-1-AP) by Proteintech is a Polyclonal antibody targeting CSF1R/CD115 in WB, IHC, ELISA applications with reactivity to mouse, rat samples 34112-1-AP targets CSF1R/CD115 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples. Tested Applications Positive WB detected in: NR8383 cells Positive IHC detected in: rat spleen tissue, mouse spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Rat CSF1R, also known as CD115, is the colony stimulating factor 1 receptor and belongs to the type III receptor tyrosine kinase family. This protein serves as a key regulator for the development, survival, proliferation, and function of the macrophage lineage. Upon binding to its ligands, CSF-1 and IL-34, it activates downstream signaling pathways, thereby maintaining the homeostasis of tissue-resident macrophages. In the nervous system, CSF1R is specifically highly expressed in microglia and is crucial for their maintenance and function. Dysregulation of this receptor is associated with various diseases, including neurodegenerative disorders, inflammatory diseases, and the modulation of the tumor microenvironment, making it an important research target and a potential biomarker.(PMID: 36335744) Specification Tested Reactivity: mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Eg6221 Product name: Recombinant rat M-CSFR/CSF1R protein Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 1-554 aa of NM_001029901.1 Sequence: MELGAPLVLLLATAWHGVQILSGEKSRTTIHIYLLAGRRAVTTEIVLMGQRSTDVQRVNDCQGAPVIEPSGPELVVEPGATVTLRCVSNGSVEWDGPISPYWTLDSESPGSILITKNATFKNTGTYRCTELEDPMRGSTAIHLYVKDPVRPWNLLAQEVTVFEGQEAVLPCLITDPTLKDSVSLVREWGRPVSRKTVYSFLPWRGFIIHKAKFLDSHTYMCKAVVNARESTSIGIRLKVNRAHPGPPHIILEPTKLVRIRGEAAQIVCSATHSEVEFNVILKRGDTKLEIPINSDFQDNAYKKVLTLNLNAVDFQDAGIYSCVANNAAGSNTATMNFQVVESAYLNLTSEQSLLQEVSVGENLDLTVIADAYPGLQRYNWTYLGPFFEDPHNLEFRTQWTTYSYSFKLHLNRVKPLEAGRYSLMAQNKAGWNNLTFELTLRYPPEVSVTWIPVNGSDVLLCDVSGYPQPNVTWMECRGHTDRCDEAQASQVWDDTQPEVLSQKPFHRVILQSQLPIGTLKHNMTYVCRAHNNVGNSSQFFRAISLGQSKQLPDE Predict reactive species Full Name: colony stimulating factor 1 receptor Calculated Molecular Weight: 114 kDa Observed Molecular Weight: 150 kDa GenBank Accession Number: NM_001029901.1 Gene Symbol: Csf1r Gene ID (NCBI): 307403 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity Purification UNIPROT ID: D4ACA7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.