Proteintech, 51009-2-AP, BRD7 Polyclonal antibody

Size: 20ul / 150ul The BRD7 (51009-2-AP) by Proteintech is a Polyclonal antibody targeting BRD7 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 51009-2-AP targets BRD7 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: COLO 320 cells, mouse cerebellum tissue, mouse lung tissue, L02 cells, rat brain tissue, SH-SY5Y cells, HeLa cells, Jurkat cells, MDA-MB-231 cells, PC-3 cells, SH-5Y5Y cells Positive IHC detected in: human colon cancer tissue, human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information BRD7 (bromodomain containing 7), is a subunit of poly-bromo-associated BRG1-associated factor (PBAF)-specific Swi/Snf chromatin remodeling complexes.BRD7 is proposed as a tumor suppressor protein, since its identification as an important component of both functional p53 and BRCA1 (breast cancer 1, early onset) pathways. BRD7 could down-regulate the GSK3beta phosphotransferase activity as dephosphorylation of GSK3B at 'Tyr-216', thus activate the Wnt signaling pathway in a DVL1-dependent manner. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3911 Product name: Recombinant human BRD7 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-353 aa of BC050728 Sequence: MGKKHKKHKSDKHLYEEYVEKPLKLVLKVGGNEVTELSTGSSGHDSSLFEDKNDHDKHKDRKRKKRKKGEKQIPGEEKGRKRRRVKEDKKKRDRDRVENEAEKDLQCHAPVRLDLPPEKPLTSSLAKQEEVEQTPLQEALNQLMRQLQRKDPSAFFSFPVTDFIAPGYSMIIKHPMDFSTMKEKIKNNDYQSIEELKDNFKLMCTNAMIYNKPETIYYKAAKKLLHSGMKILSQERIQSLKQSIDFMADLQKTRKQKDGTDTSQSGEDGGCWQREREDSGDAEAHAFKSPSKENKKKDKDMLEDKFKSNNLEREQEQLDRIVKESGGKLTRRLVNSQCEFERRKPDGTTTLGL Predict reactive species Full Name: bromodomain containing 7 Calculated Molecular Weight: 651 aa, 74 kDa Observed Molecular Weight: 70-74 kDa GenBank Accession Number: BC050728 Gene Symbol: BRD7 Gene ID (NCBI): 29117 RRID: AB_2259226 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NPI1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.