Proteintech, 51062-1-AP, PHF6 Polyclonal antibody

Size: 20ul / 150ul The PHF6 (51062-1-AP) by Proteintech is a Polyclonal antibody targeting PHF6 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 51062-1-AP targets PHF6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells Positive IHC detected in: human placenta tissue, human cervical cancer tissue, human ovary tumor tissue, human stomach cancer tissue, mouse brain tissue, mouse stomach tissue, rat stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:20000-1:100000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information PHF6 protein is highly conserved in vertebrates. Structurally, PHF6 protein contains two plant homeodomain (PHD)-like zinc fingers, two nuclear localization sequences and a nucleolar localization sequence, suggesting that PHF6 may play a role in the regulation of transcription. PHD zinc fingers are structurally conserved modules that interact with chromatin or mediate protein-protein interactions. PHF6 associates with subunits of the PAF1 transcription elongation complex including PAF1, LEO1, CTR9 and CDC73.The interaction of PHF6 with the PAF1 complex plays a critical role in PHF6 function in neuronal migration in the cerebral cortex. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2102 Product name: Recombinant human PHF6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-312 aa of BC005994 Sequence: MSSSVEQKKGPTRQRKCGFCKSNRDKECGQLLISENQKVAAHHKCMLFSSALVSSHSDNESLGGFSIEDVQKEIKRGTKLMCSLCHCPGATIGCDVKTCHRTYHYHCALHDKAQIREKPSQGIYMAYCRKHKKTAHNSEAADLEESFNEHELEPSSPKSKKKSRKGRPRKTNFKGLSEDTRSTSSHGTDEMESSSYRDRSPHRSSPSDTRPKCGFCHVGEEENEARGKLHIFNAKKAAAHYKCMLFSSGTVQLTTTSRAEFGDFDIKTVLQEIKRGKRMVCSFYICYATLHLICCFKFRVHPKFIQSSENLK Predict reactive species Full Name: PHD finger protein 6 Calculated Molecular Weight: 365aa,41 kDa; 312aa,35 kDa Observed Molecular Weight: 41 kDa GenBank Accession Number: BC005994 Gene Symbol: PHF6 Gene ID (NCBI): 84295 RRID: AB_2165369 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8IWS0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.